AIN573 - Analytical Instrumentation

Subject Title
Analytical Instrumentation

Subject Description
This subject offers both lecture and laboratory components. This is an analytical chemistry course in which modern automated gas chromatographs with mass selective detectors, GC/MSD, and high performance liquid chromatographs, HPLC, are used for the quantitative determination of sample components. All HPLC and GC/MSD instruments are equipped with Agilent ChemStation software. Students create their own methods and carry out data analysis independently. Standard methods of analysis using an external standard calibration curve and the use of internal standards are incorporated into the chosen labs. Students prepare their own solutions and analyse unknowns independently. Wavelength selection for the detection of eluates in HPLC, the relationship between ? max and optimisation of the signal as well as terms relevant to accurate quantitation work are covered.

Credit Status
Worth one credit towards BTA program, Semester 4

Learning Outcomes
Upon successful completion of this subject the student will be able to:

• Interpret structural formulas of organic compounds with a variety of functional groups.
• Compare the physical properties of different organic compounds based on the functional groups that are present and the preferred conformation of the compound.
• Relate the electronic properties of a molecule to the data provided by selected molecular spectroscopy techniques.
• Acquire and interpret data from an Infrared Spectrometer
• Analyze and interpret a ¹H NMR spectrum
• Explain the basic instrumentation in a mass spectrometer coupled with a gas chromatographic system.
• Explain the basic theory of an electron ionisation mass spectrometer and detection by a quadrupole mass analyzer.
• Perform a qualitative analysis of an organic mixture in a mass spectrometer coupled with a gas chromatographic system.
• Explain the meaning of SCAN and SIM modes in mass spectral analysis.
• Explain the meaning of a total ion chromatogram in mass spectral analysis.
• Generate a total ion chromatogram from mass spectral data.
• Perform qualitative identification of a molecule by library search in mass spectral analysis.
• Explain the meaning of and the use of an extracted ion chromatogram in mass spectral analysis.
• Generate an extracted ion chromatogram in mass spectral analysis.
• Check the validity of a library identification by isotopic abundance ratio.
• Explain the basic principles of an electron spray ionization mass spectrometry.
• Operate Unicam UV/VIS Spectrophotometer, Hewlett Packard liquid chromatograph with Hewlett Packard 1050 &1200 software, Hewlett Packard HP5890 series II, gas chromatograph with HP5971 MSD
• Know the theory of molecular spectroscopy of UV-VIS absorption and it’s applications in biochemical analysis.
• Utilize the relationship between absorbance, concentration and path length of Beer-Lambert law in quantitative analysis.
• Know the basic theory of GC-MS ( EID) and LC-MS ( Electrospray).
• Run SIM mode on a GC/MSD and quantitate trace contaminant(s) in a given unknown sample.
• Perform accurate analytical preparations.
• Record and report scientific data following the principles of Good Laboratory Practice.
• Locate and adhere to accepted safety practices while performing laboratory work (WHMIS, MSDS).
• Recall the limitations of methods and instruments used in the course

Essential Employability Skills

    •  Communicate clearly, concisely and correctly in the written, spoken and visual form that fulfils the purpose and meets the needs of the audience.

    •  Respond to written, spoken, or visual messages in a manner that ensures effective communication.

    •  Apply a systematic approach to solve problems.

    •  Analyze, evaluate, and apply relevant information from a variety of sources.

Academic Integrity
Seneca upholds a learning community that values academic integrity, honesty, fairness, trust, respect, responsibility and courage. These values enhance Seneca's commitment to deliver high-quality education and teaching excellence, while supporting a positive learning environment. Ensure that you are aware of Seneca's Academic Integrity Policy which can be found at: http://www.senecapolytechnic.ca/about/policies/academic-integrity-policy.html Review section 2 of the policy for details regarding approaches to supporting integrity. Section 2.3 and Appendix B of the policy describe various sanctions that can be applied, if there is suspected academic misconduct (e.g., contract cheating, cheating, falsification, impersonation or plagiarism).

Please visit the Academic Integrity website http://open2.senecac.on.ca/sites/academic-integrity/for-students to understand and learn more about how to prepare and submit work so that it supports academic integrity, and to avoid academic misconduct.

Discrimination/Harassment
All students and employees have the right to study and work in an environment that is free from discrimination and/or harassment. Language or activities that defeat this objective violate the College Policy on Discrimination/Harassment and shall not be tolerated. Information and assistance are available from the Student Conduct Office at student.conduct@senecapolytechnic.ca.

Accommodation for Students with Disabilities
The College will provide reasonable accommodation to students with disabilities in order to promote academic success. If you require accommodation, contact the Counselling and Accessibility Services Office at ext. 22900 to initiate the process for documenting, assessing and implementing your individual accommodation needs.

Camera Use and Recordings - Synchronous (Live) Classes
Synchronous (live) classes may be delivered in person, in a Flexible Learning space, or online through a Seneca web conferencing platform such as MS Teams or Zoom. Flexible Learning spaces are equipped with cameras, microphones, monitors and speakers that capture and stream instructor and student interactions, providing an in-person experience for students choosing to study online.

Students joining a live class online may be required to have a working camera in order to participate, or for certain activities (e.g. group work, assessments), and high-speed broadband access (e.g. Cable, DSL) is highly recommended. In the event students encounter circumstances that impact their ability to join the platform with their camera on, they should reach out to the professor to discuss. Live classes may be recorded and made available to students to support access to course content and promote student learning and success.

By attending live classes, students are consenting to the collection and use of their personal information for the purposes of administering the class and associated coursework. To learn more about Seneca's privacy practices, visit Privacy Notice.